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E-MTAB-2160
Pseudomonas aeruginosa
4 Downloadable Samples
Affymetrix Pseudomonas aeruginosa Array (paeg1a)
Description
In this experiment, we investigated how CbrA contributed to the expression of P. aeruginosa virulence factors in vivo using microarrays. Two independent microarray analyses were performed to identify the global gene expression of the cbrA mutant in comparison to PA14 wild type strain during D. discoideum infection.
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View Samples- Pseudomonas aeruginosa
- 4 Downloadable Samples
- Affymetrix Pseudomonas aeruginosa Array (paeg1a)
Description
In this experiment the transcriptional profile of the Pseudomonas aeruginosa PA14 two-component sensor kinase PA4398 was investigated under swarming conditions using DNA microarrays. To this aim three independent cultures of the PA14 wild-type and the PA4398 mutant were grown until mid-log phase in Luria-Bertani broth following an incubation on BM2-swarm plates containing 0.1 % (wt/vol) casaminoacids and 0.5 % (wt/vol) agar for 20 h at 37 °C. Subsequent total RNA was extracted from the leading edge of dendritic swarm colonies and analyzed by microarrays.
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View Samples- Pseudomonas aeruginosa
- 4 Downloadable Samples
- Affymetrix Pseudomonas aeruginosa Array (paeg1a)
Description
In this experiment the transcriptional response of the opportunistic human pathogen Pseudomonas aeruginosa towards physiological concentrations of the major human host defense peptide LL-37 was investigated using microarrays. To this aim, three independent cultures of P. aeruginosa PAO1 were grown until mid-log phase in Mueller-Hinton broth and subsequently incubated with either sublethal LL-37 concencentrations (20 µg/ml) or without peptide for 2 h at 37 °C following RNA extraction and microarray analysis.
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Compound
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