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GSE55222
Arabidopsis thaliana
6 Downloadable Samples
Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
We profiled transcripts from sorted phloem cells of wild-type and apl mutants to identify the genes regulated by APL in phloem.
Publication Title
Plant development. Arabidopsis NAC45/86 direct sieve element morphogenesis culminating in enucleation.
Sample Metadata Fields
Specimen part
View Samples- Mus musculus
- 12 Downloadable Samples
- Affymetrix Mouse Genome 430 2.0 Array (mouse4302)
Description
MEFs were stimulated for 6 h with IFNa or IFNg after pretreatment with AMN107 or DMSO for at least 18 h.
Publication Title
No associated publication
Sample Metadata Fields
Cell line
View Samples- Homo sapiens
- 6 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
HPSE plays important roles in gastric cancer cell proliferation, apoptosis and metastasis.The aim of this study is to explore molecular mechanism underling roles of HPSE in gastric cancer cell proliferation, survival, migration and metastasis.
Publication Title
No associated publication
Sample Metadata Fields
Disease, Disease stage, Cell line
View Samples- Mus musculus
- 4 Downloadable Samples
- Affymetrix Mouse Genome 430 2.0 Array (mouse4302)
Description
Aortic aneurysm is a life-threatening cardiovascular disorder due to the predisposition for dissection and rupture. Genetic studies have proved the involvement of the transforming growth factor- (TGF-) pathway in aortic aneurysm. Smad4 is the central mediator of canonical TGF- signaling. However, the exact role of Smad4 confined to the smooth muscle cells (SMCs) in the pathogenesis of aortic aneurysm is largely unknown. Furthermore, whether TGF- signaling disruption in SMCs could directly trigger aortic wall inflammation remains poorly investigated. Recently, we revealed a pivotal role of smooth muscle Smad4 signaling in maintaining aortic wall homeostasis and protecting against the development of aortic aneurysm and dissection. To evaluate the underlying mechanism by which Smad4 regulate VSMC functions and affects aneurysm formation and development, Smooth muscle specific Smad4 Knockout mice and the control littermate were sacrificed at 6 weeks old, and their aortic ateries were collected.We combined 3-5 vessels for one sample, and 2 samples for each phenotype. Subsequently, a total of 400ng RNA was used following Affymetrix instruction and 2 ug of cRNA were hybridized for 16 hr at 45. GeneChips were scanned using the Scanner 7G and the data was analyzed with Expression Console using Affymetrix default analysis settings and global scaling as normalization method. RMA analysis was employed to evaluate the gene expression.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 2 Downloadable Samples
- Affymetrix Mouse Genome 430 2.0 Array (mouse4302)
Description
TGF-beta/Smads signaling plays important roles in vascular integrity. To identify potential Smad4 target genes in brain endothelial cells that control cerebrovascular integrity, the microarray assay was performed to compare the gene expression profiles of bEnd3 transfected with Smad4-siRNA and control-siRNA.
Publication Title
Endothelial Smad4 maintains cerebrovascular integrity by activating N-cadherin through cooperation with Notch.
Sample Metadata Fields
Specimen part, Cell line
View Samples- Homo sapiens
- 3 Downloadable Samples
Illumina HiSeq 4000
Description
Cervical cancer cell line C33A
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Disease stage, Cell line, Treatment, Race
View Samples- Mus musculus
- 3 Downloadable Samples
- NextSeq 500
Description
In the terms of vaccine efficacy and duration of protection in malaria vaccination is major concern against malaria. On the other hand, it is facing complications in development and administration to the host. However, whole sporozoites vaccination (WSV) is far more efficacious than any other alternative strategy. We have found that the intermittent sporozoite challenge to immune mice following RAS vaccination extends the longevity of sterile protection by maintaining CD8+T cell memory responses to LS infection and also helps in CD8a+DCs accumulation and activation in liver. Consequently, there has been great interest in elucidating and understating the sterile immunological response at mechanistic level. The information we have generated can then potentially be used in generation of next generation vaccine with improved efficacy and duration of protection. In this work, to elucidate the host initial immune response underlying the protective effects of a WSV in shaping the protected sterile protection and advances its immunogenicity in the future, a high-throughput RNA sequencing technology was used to investigate the immunization related gene expression patterns of mouse immunized with radiation attenuated sporozoites (RAS) vaccine.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line, Treatment
View Samples- Arabidopsis thaliana
- 2 Downloadable Samples
- Illumina HiSeq 2500
Description
Introduction: The Plant Organelle RNA Recognition (PORR) domain proteins are nucleus-encoded RNA-binding proteins that have acquired specific roles in organelle RNA metabolism as splicing factors of chloroplast group II introns. LEFKOTHEA (At5g62990) is a nuclear gene encoding a PORR domain protein that carries a transit peptide (TP) and monopartite or bipartite nuclear localization signals (NLS). These motifs result in dual-targeting of LEFKOTHEA to the nucleus and chloroplasts implying a role in the splicing of chloroplast group II introns and nuclear pre-mRNA introns. Therefore, we examined the splicing efficiency of plastid and nuclear genes in lefko2 mutant.Methods: The lefko2 mutant was isolated from a genetic screen of an M2 EMS-mutagenized Arabidopsis thaliana Columbia (Col-0) background seed population. The lefko2 mutant allele has a white cotyledon phenotype caused by a G to A mutation in the coding region resulting in a Glycine (G) 373 to Aspartic acid (D) conversion. Total RNA was extracted using plant RNA kit spin columns with an on-column DNase treatment from lefko2 mutant and wild-type Arabidopsis cotyledons. The quantity and integrity of the RNA was assessed using a NanoDrop 1000 spectrophotometer and agarose gel electrophoresis. RNA-seq libraries were generated using the TruSeq Low Input kit according to the manufacturer's instructions (Illumina). Sequencing was performed on an Illumina HiSeq 2000 instrument at BGI (Beijing Genomics Institute). Raw reads were filtered into clean reads and aligned to the Arabidopsis genome (TAIR10). RNA-seq data were analyzed using the SOAP (Release 2.121) with parameters “-s 40 -l 32 -v 3 -r 2” and the TopHat/Cufflinks pipeline (version 2) with parameters “-p 16 --solexa1.3-quals --segment-length 30 --segment-mismatches 2 -r 20 --mate-std-dev 20 --library-type fr-unstranded”. We generated 480 million paired-end reads (101 bp in length) for each sample. On average, about 90% of these reads could be unambiguously aligned to the TAIR10 reference genome sequence. Alternative splicing events were detected for lefko2 and wild-type using Spladder. Intron retention events detected in wild-type were filtered out from lefko2 or vice-versa, and visualized using the Integrative Genomics Viewer (IGV) tool.Results: Splicing defects were observed in numerous nuclear genes of lefko2 cotyledons compared to wild type. Among them, intron retention (IR) events were the most prominent. Further, the fidelity of 5' splice site (5'SS) donor and 3'SS acceptor splicing was disturbed in lefko2 cotyledons. To less extend, exon skipping (ES) defects were also detected.Conclusions: Detailed nuclear splicing events were widely observed in lefko2 cotyledons demonstrating a prevalent role of LEFKOTHEA in the splicing of nuclear pre-mRNA introns.Overall design: RNA-seq libraries were generated using the TruSeq Low Input kit according to the manufacturer's instructions (Illumina). Sequencing was performed on an Illumina HiSeq 2500 instrument at BGI (Beijing Genomics Institute).
Publication Title
No associated publication
Sample Metadata Fields
Age, Specimen part
View Samples- Pseudomonas aeruginosa
- 1 Downloadable Sample
- Illumina HiSeq 2500
Description
The study was conducted to understand the effect of Solanum torvum root extract upon Pseudomonas aeruginosa signaling system . Extract could suppress quorum sensing genes due to which the bacteria remains attenuated inside a host.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Saccharomyces cerevisiae
- 16 Downloadable Samples
- Affymetrix Yeast Genome 2.0 Array (yeast2)
Description
Effect of FLO8 or MSS11 deletion and -overexpression on yeast transcript profiles compared to wild type in laboratory yeast strains 1278b and S288c.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples