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SRP091899
Rattus norvegicus
4 Downloadable Samples
Illumina HiSeq 2500
Description
This is a whole transcriptome sequencing data of rat testis. YY1 gene was knocked down in Experimental animals under Sertoli cell specific and puberty specific promoter. These knockdown animals were compared with the control animals.
Publication Title
An integrated transcriptomics-guided genome-wide promoter analysis and next-generation proteomics approach to mine factor(s) regulating cellular differentiation.
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 9 Downloadable Samples
- Illumina HiSeq 4000
Description
To get a deeper understanding of molecular mechanism elicited by alogliptin intervention, high-throughput RNA sequencing (RNA-seq) of the expression levels of all genes in the liver was performed
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples SRP168448- Mus musculus
- 12 Downloadable Samples
- Illumina HiSeq 3000
Description
The expression of lncRNAs in the hypothalamic neuronal stem cells of young mice and aged mice.The expression pattern of mRNAs in the hypothalamic neuronal stem cells of aged Hnscr null mice and littermate wild-type mice.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line, Treatment
View Samples- Mus musculus
- 6 Downloadable Samples
- Illumina HiSeq 2500
Description
Statins, the cholesterol lowering agents, can increase diabetes incidence and impair glucose tolerance via its detrimental effects on non-hepatic tissues, such as pancreatic islet, but underlying mechanism has not been clarified. In atorvastatin-treated high fat diet mice, we found reduced pancreatic ß-cell size, ß-cell mass, mature insulin granules and reduced insulin secretion along with the deteriorated glucose tolerance. Transcriptome profiling of primary pancreatic islets showed inhibitory effects of atorvastatin on expression of genes encoding key pancreatic transcription factors, mTOR signaling pathway and small G proteins (sGPs).
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Cell line, Treatment
View Samples- Homo sapiens
- 6 Downloadable Samples
- Illumina HiSeq 2500
Description
We found the feasibility of targeting TNIK in osteosarcoma. Here, we describe the effects of pharmacological inhibition of TNIK in osteosarcoma cell lines.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Cell line, Treatment
View Samples- Danio rerio
- 14 Downloadable Samples
- IlluminaHiSeq1000
Description
RNA-seq data from control and MCT8 morphant zebrafish embryos at 25hpf
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 120 Downloadable Samples
- IlluminaHiSeq2500
Description
In current study, we performed 12-weeks time-course mRNA expression analysis on the biological sextuplicate samples of estrogen receptor (ER)-positive MCF-7 breast cancer cells in presence or absence of tamoxifen to capture cellular state changes associated with acquisition of tamoxifen resistance. mRNA (1 mg) obtained from the MCF-7 cells was used for Poly A+ mRNA-sequence using the Illumina TruSeq RNA Library Prep Kit v2 according to the manufacturer protocol. 100 base pair-end reads or 36 base single-end-reads were obtained using Hiseq2500 (Illumina) and analyzed by analysis software provided by Illumina. To ensure the validity of the experiment, expression of representative genes (such as EGFR, ErbB2 (HER2), IGF-IR, NCOA3 (AIB1), MYC, CCND1 (cyclin D1) and CCNE1) known for tamoxifen resistance in vitro and clinical setting (Musgrove 2009) were confirmed.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples SRP188296- Homo sapiens
- 31 Downloadable Samples
- Illumina HiSeq 2500
Description
RNA-seq involves purification of RNA, followed by either selection of poly-A(+) RNA or depletion of ribosomal RNA. RNA is then converted into cDNA by one of two methods; 1) random priming, followed by cDNA fragmentation, end-repair and Illumina/SOLiD linker ligation or, 2) Enzymatic or chemical RNA fragmentation followed by linker ligation and cDNA generation. Following PCR amplification of tailed cDNA fragments with primers suitable for solid phase (Illumina) or emPCR (SOLiD) clonal amplification RNA-seq libraries are subjected to sequencing. Sequence alignment software is then used to compare the short sequence reads to reference genome and transcriptome databases, and exon-exon junction databases. From this analysis paradigm emerges data that is used for a variety of purposes, including the measurement of gene- level and exon-level expression abundance; detection of base changes (mutations and polymorphisms) relative to reference datasets; measurement of alternative splicing events; identification of gene fusion events; and identification of RNA editing events.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Race
View Samples- Rattus norvegicus
- 36 Downloadable Samples
Affymetrix Rat Genome 230 2.0 Array (rat2302)
Description
This SuperSeries is composed of the SubSeries listed below.
Publication Title
Differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation.
Sample Metadata Fields
Specimen part, Cell line
View Samples- Rattus norvegicus
- 12 Downloadable Samples
- Affymetrix Rat Genome 230 2.0 Array (rat2302)
Description
Cell substrate adhesion plays an important role in cellular transformation of rat fibroblast cell lines, however a few viable non-adherent fibroblast cells when placed in suspension for a time period of 16 h (NA16) showed varied phenotypic characteristics like colony and tumor formation
Publication Title
Differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation.
Sample Metadata Fields
Specimen part, Cell line
View Samples